グループIIのイントロンRNAは,イントロンの移動に関与するDNAエンドヌクレアースの触媒成分です
1Department of Molecular Genetics, Ohio State University, Columbus 43210-1292, USA.
Cell
|November 17, 1995
まとめ
イーストミトコンドリア群IIイントロンAl2の移動にはリボジーム複合体が含まれています. この複合体は,al2 RNAとタンパク質で構成され,DNAを二重鎖の断裂で割って,イントロンホーミングを可能にします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- グループIIイントロンは,様々な生物における移動性遺伝的要素である.
- イントロン移動,またはホーミングは,しばしば反転スプライシングと反転転写によって媒介されます.
- イーストミトコンドリア群IIイントロンal2は,ホーミングのためのDNAプリムリバーストランスクリプションを使用しています.
研究 の 目的:
- al2 intron homingの間に二重鎖の断裂形成のメカニズムを解明する.
- al2イントロンでコードされたタンパク質とRNAの酵素活性性を特徴づける.
- DNA分裂におけるリボヌクレオプロテイン複合体の役割を調査する.
主な方法:
- DNAの分裂活動を分析するための生化学的分析.
- リボヌクレオプロテイン複合体の構成の特徴.
- DNA基板とのRNAとタンパク質の相互作用に関するインビトロ研究.
主要な成果:
- 二重鎖の断裂の原因となるDNA内核酵素は,al2RNAとタンパク質のリボ核タンパク質複合体である.
- al2RNA成分は,部分的逆接合反応によって感覚鎖DNAの分裂を触媒化する.
- al2タンパク質成分は,反感覚DNA鎖を割って活性イントロン構造を安定させるように見える.
結論:
- al2イントロンは,DNA分裂のための新しいリボ酵素機構を使用し,カノンカルRNAスプライシングとは異なる.
- リボヌクレオプロテイン複合体は,サイト特異のDNAエンドヌクレアースとして作用し,イントロンホーミングを容易にします.
- この研究は,DNA基板を含む生物学的に重要なリボジーム反応を明らかにしています.
関連する概念動画
The Nucleus
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Nucleic Acids
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
The Nucleus
The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
DNA has a double-helix structure. The...


