ゴルギ複合体を通る交通に必要なSNAREのようなタンパク質です
D K Banfield1, M J Lewis, H R Pelham
1MRC Laboratory of Molecular Biology, Cambridge, UK.
Nature
|June 29, 1995
まとめ
研究者は,分泌経路に関与する新しい酵母タンパク質を特定しました. v-SNAREに似たタンパク質であるSft1pは,ゴルギ装置内の輸送に不可欠であり,単一のt-SNAREが複数のステップを調節できることを示唆しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- ユカリオットの分泌経路は,連続した膜に結合したコンパートメントを含む.
- 輸送ベシクルは,これらのコンパートメント間の貨物の移動を媒介する.
- 溶性NSF結合タンパク質受容体 (SNAREs) は,膀の標的化と融合に不可欠である.
研究 の 目的:
- 分泌経路内の膀輸送に関与する新しいタンパク質を特定する.
- Sed5pと相互作用する酵母膜タンパク質の機能を調査するために,ER-Golgi t-SNARE.
主な方法:
- イーストにおける遺伝子相互作用の研究.
- タンパク質の構造的類似性分析.
- 細胞内輸送における特定されたタンパク質の機能分析.
主要な成果:
- 2つの酵母膜タンパク質が,Sed5p.との遺伝的相互作用と特定されました.
- 特定されたタンパク質の1つであるSft1pは,構造的にはv-SNAREsに類似しています.
- Sft1pは,初期のゴルギ区間と後のゴルギ区間間の輸送に不可欠です.
結論:
- 単一のt-SNARE (Sed5p) は,分泌経路における複数の輸送ステップを調節することができる.
- Sft1pは,ゴルギ内交通に関与するSNARE候補である.
- この研究は,ゴルギの密輸の分子メカニズムに関する新しい洞察を提供します.
関連する概念動画
01:49Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
01:43SNAREs and Membrane Fusion
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:12Golgi Matrix Proteins
Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
01:28Protein Translocation Machinery on the ER Membrane
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...