染色体組立因子Iのp150およびp60サブユニット:新たに合成されたヒストンとDNA複製の間の分子リンク
P D Kaufman1, R Kobayashi, N Kessler
1Cold Spring Harbor Laboratory, New York 11724, USA.
Cell
|June 30, 1995
まとめ
染色体組み立て因子I (CAF-I) は,複製DNAにヒストンを組み込むために不可欠なタンパク質複合体です. p150およびp60サブユニットは,この重要なプロセスを促進するために相互作用し,新たに合成されたヒストンと複合体を形成します.
科学分野:
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
- 細胞生物学 細胞生物学
背景:
- 染色体組立因子I (CAF-I) は,DNA複製と核細胞形成に不可欠な複合体である.
- CAF-Iのメカニズムを理解することは,ゲノム安定性と遺伝子調節を理解するための鍵です.
研究 の 目的:
- クロマチン組立におけるヒトCAF-Iの分子機構とサブユニット相互作用を解明する.
- DNA複製中のヒストンの堆積におけるp150およびp60サブユニットの役割を特定する.
主な方法:
- CAF-Iサブユニット (p150およびp60) のcDNA配列の分析.
- p150とp60のタンパク質相互作用を調査する.
- CAF-Iのクロマチン組立機能に対するドメイン削除の影響を評価する.
- CAF-Iと新たに合成されたヒストンを含むタンパク質複合体を特定する.
主要な成果:
- 人間のCAF-Iは3つのサブユニットからなる複合体で,複製DNAへのヒストンオクトーマー組成に不可欠です.
- p150サブユニットは充電された残留物を持ち,p60はWDリピートを含んでいる.
- p150とp60は直接相互作用し,複製に依存する核細胞組成には不可欠である.
- p150のp60結合ドメインを削除すると,クロマチンの組立活動が廃止されます.
- CAF-Iは,新たに合成されたヒストーンH3とアセチル化ヒストーンH4と複合体を形成する.
結論:
- CAF-Iのp150およびp60サブユニットは,DNA複製結合クロマチンの組立に不可欠です.
- CAF-Iは,複製DNAに蓄積される前に,新たに合成されたヒストンと相互作用することによって機能する可能性があります.
関連する概念動画
02:31Chromosome Replication
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins. This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
02:05Duplication of Chromatin Structure
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
01:32Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...