関連する実験動画
Updated: Jul 5, 2026
09:58
RhoC GTPase Activation Assay
Published on: August 22, 2010
RhoファミリーのGTPases RhoA,Rac1,CDC42Hsは,SRFによる転写活性化を調節する
C S Hill1, J Wynne, R Treisman
1Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, England.
Cell
|June 30, 1995
まとめ
血清応答因子 (SRF) は,Rho媒介信号の核標的である. この経路は,MAPキナーゼ経路とは異なるc-fosプロモーター活性を調節する経路である.
科学分野:
- 分子生物学は分子生物学である.
- 細胞シグナリング 細胞シグナリング
背景:
- c-fos血清応答要素 (SRE) は,転写因子SRFとTCFを結合する.
- SRFだけで,血清,LPA,またはGタンパク質シグナル伝達に反応して転写を活性化します.
研究 の 目的:
- SRF媒介による転写におけるRho GTPasesの役割を調査する.
- SREでRhoシグナリングがTCFシグナリングと収束するかどうかを判断する.
主な方法:
- SRF媒介による転写に対するRho GTPases (RhoA, Rac1, CDC42Hs) の影響を調査した.
- 特定のシグナリング刺激に対する機能的Rhoの要件を評価した.
- SRF経路の活性化とMAPキナーゼの活性化との相関を調べました.
主要な成果:
- 活性化されたRho GTPasesは,SREのTCF活性化剤と連携する.
- 機能的Rhoは,特定の刺激によってSRFへのシグナル伝達に不可欠ですが,すべてではありません.
- SRF経路の活性化は,ERK,SAPK/JNK,MPK2/p38の活性化とは独立しています.
- 機能的なRhoは,c-fosプロモーターの活性を調節するために必要である.
結論:
- SRFは,新しいRho媒介シグナル伝達経路の核標的である.
- RhoGTPaseは,SRF経由で遺伝子の転写を調節する上で重要な役割を果たします.
さらに関連する動画
11:28Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates
Published on: March 31, 2012
13:51Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
Published on: November 11, 2018
関連する概念動画
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...
01:21Cell Polarization by Rho Proteins
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
01:24Small GTPases - Ras and Rho
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
01:07MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
01:22Activation and Inactivation of G Proteins
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...