14-3-3タンパク質のゼータイソフォームの結晶構造は14-3-3タンパク質のゼータイソフォームの結晶構造である
D Liu1, J Bienkowska, C Petosa
1Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Nature
|July 13, 1995
まとめ
14-3-3タンパク質ファミリーは,細胞内信号伝達を調節する. 研究者は,主要な14-3-3タンパク質の結晶構造を決定し,多様な細胞相互作用のための保存された結合溝を明らかにしました.
科学分野:
- 分子生物学は分子生物学である.
- 構造生物学 構造生物学とは
- バイオケミストリー バイオケミストリー
背景:
- 14-3-3タンパク質は,細胞内信号伝達経路の重要な調節体である.
- 腫瘍遺伝子の産物や原発がん遺伝子の産物と相互作用し,細胞のプロセスに影響を与えます.
- 以前の研究では,キナーゼを活性化し,細胞の成熟を誘発する役割が示されていた.
研究 の 目的:
- 主要哺乳類の14-3-3タンパク質イソフォームの結晶構造を決定する.
- 14-3-3タンパク質が他の細胞分子と相互作用する構造的基礎を解明する.
主な方法:
- タンパク質の構造を決定するために,X線結晶学を用いた.
- 達成された解像度は2.9Angstromsでした.
主要な成果:
- 結晶構造は,サブユニットごとに9つの反並列ヘリクスを含む二次元タンパク質を明らかにしました.
- 追加のヘリクスを結合できる中央アンフィパティック・グルーヴが特定されました.
- ダイマー界面とリガンド結合表面の保存された残留物は,種ごとに観察されました.
結論:
- 特定された溝は,さまざまな細胞パートナーと14-3-3タンパク質の相互作用の一般的なメカニズムを示唆しています.
- 脊椎動物,酵母,植物における14-3-3タンパク質の構造的・機能的保存が示されています.
さらに関連する動画
関連する概念動画
01:28Protein Organization
Proteins are one of the fundamental building blocks of life that carry out many diverse functions in the cell. Proteins are assembled from amino acids. The sequence of amino acids is known as the primary structure of a protein. Local interactions of individual amino acids cause the linear chain to fold into the secondary structures. Interactions of distant amino acids lead to further folding of the protein—the tertiary structure. The assembly of multiple folded chains (subunits) is known as...
01:49Protein Folding
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation which is critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.Protein Structure Is Critical to Its Biological FunctionProteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
02:39Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.A protein's shape is critical to its function. For example, an enzyme can...
01:28Protein Organization
Proteins are one of the fundamental building blocks of life that carry out many diverse functions in the cell. Proteins are assembled from amino acids. The sequence of amino acids is known as the primary structure of a protein. Local interactions of individual amino acids cause the linear chain to fold into the secondary structures. Interactions of distant amino acids lead to further folding of the protein—the tertiary structure. The assembly of multiple folded chains (subunits) is known as...
01:24Protein Organization
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
The primary structure of a protein is its amino acid sequence.
01:25Protein Folding
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...