マウスのEGF受容体の標的型破壊:遺伝子背景が突然変異したフェノタイプに与える影響
D W Threadgill1, A A Dlugosz, L A Hansen
1Department of Genetics, Case Western Reserve University, Cleveland, OH 44106-4955, USA.
Science (New York, N.Y.)
|July 14, 1995
まとめ
遺伝子ターゲティングにより,表皮成長因子受容体 (EGFR) のゼロアレルが生成されました. EGFR欠乏症は,遺伝的背景によって重度が異なる発達障害と胚死亡を引き起こしました.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- 皮膚表皮成長因子受容体 (EGFR) は,細胞の成長と分化に不可欠です.
- EGFRの役割を理解するには,その機能を in vivo で研究する必要があります.
研究 の 目的:
- エピデルマ・成長因子受容体 (EGFR) のインビボ機能を調査する.
- 異なる遺伝的背景におけるEGFR欠乏の発達上の影響を特徴づける.
主な方法:
- 遺伝子のターゲティングは,表皮成長因子受容体 (Egfr) のゼロアレルを生成するために使用されました.
- ホモジゴス型変異性マウスのフェノタイプ分析は,様々な遺伝的背景 (CF-1,129/Sv,CD-1) に基づいて行われました.
主要な成果:
- EGFR欠乏症は,内部の細胞質の変性によるCF-1の背景でのペリインプラント死亡率につながった.
- 129/Svの背景では,同卵性変異体は胎盤の欠陥による妊娠中期の死亡率を示した.
- CD-1の背景では,突然変異者は3週間まで生存し,皮膚,腎臓,脳,肝臓,胃腸に異常を示した.
結論:
- EGFRは胚の発達に不可欠であり,その機能は遺伝的背景に非常に敏感である.
- EGFRは,複数の臓器系において重要な役割を果たし,細胞活動への広範な関与を強調しています.
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