C-Cケモカイン受容体の分子クローン,機能表現,シグナル伝達特性
K Neote1, D DiGregorio, J Y Mak
1Department of Immunology, Genentech, Incorporated, South San Francisco, California 94080.
Cell
|February 12, 1993
まとめ
研究者らは,C-Cケモカイン受容体1 (C-C CKR-1) を特定し,これはC-Cケモカインを結合するタンパク質である. この受容体は免疫反応に役割を果たし,ウイルス免疫に関与している可能性があります.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
背景:
- C-Cケモカインは,免疫細胞の密輸の重要な調節因子である.
- これらのタンパク質は,リンパ球,単細胞,エオシノフィル,およびベソフィルを惹きつけ,免疫反応に影響を与えます.
研究 の 目的:
- C-Cケモカインの新しい受容体を特定し,特徴づけること.
- C-Cケモカインの受容体との結合親和性とシグナル伝達能力を調査する.
- ウイルス免疫におけるC-Cケモカインの潜在的役割を調査する.
主な方法:
- 7つの膜を横断する受容体,C-C CKR-1をコードするcDNAの分離.
- 様々なC-Cケモカイン (MIP-1α,MCP-1,RANTES,MIP-1β) のC-C CKR-1への結合を評価する.
- 受容体シグナル伝達を評価するために細胞内カルシウム流量を測定する.
- C-Cケモカインと,サイトメガロウイルスにコードされたC-C CKR-1関連遺伝子産物との相互作用を調査する.
主要な成果:
- 新しい受容体,C-C CKR-1が特定され,特徴づけられました.
- C-C CKR-1は,ヒトおよびネズミのマクロファージ炎症タンパク質1α (MIP-1α),ヒト単細胞化学反応タンパク質1 (MCP-1),およびRANTESを様々な親和で結合する.
- ケモカイン結合親和性は,細胞内カルシウム流動によって示されたように,信号伝達効率と相関しませんでした.
- C-Cケモカインは,サイトメガロウイルスにコードされたC-C CKR-1に関連するタンパク質と結合することが判明しました.
結論:
- C-C CKR-1は,複数のC-Cケモカインの機能的受容体である.
- 差異的なシグナル応答は,複雑な規制メカニズムを示唆しています.
- C-Cケモカインは,ウイルス遺伝子産物との相互作用を通じて,ウイルス免疫に役割を果たす可能性があります.
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