人間のTAFII250のクローン化と発現:細胞サイクル調節に関与するTBP関連因子
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Nature
|March 11, 1993
まとめ
研究者らは,遺伝子転写の調節に不可欠な重要なコアクティベータータンパク質であるhTAFII250を特定しました. このTBP関連因子 (TAF) は,転写制御と細胞サイクル進行を結びつける.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- 細胞生物学 細胞生物学
背景:
- RNAポリメラーゼIIによる基礎転写には補助因子 (TFIIA-J) が必要です.
- 同活性化剤は,転写活性化剤の調節には重要ですが,基礎転写には欠かせません.
- 一部のコアクティベーターは,TATA結合タンパク質 (TBP) と結合し,TFIID複合体を形成する.
研究 の 目的:
- TBPとTBP関連因子 (TAF) の関係を定義する.
- ヒト初のTAFであるhTAFII250.0.をクローン化,発現し,特徴づけること.
主な方法:
- 再結合 hTAFII250.0.のクローン化と発現
- TBPの相互作用を評価するためのインビトロおよび酵母ベースの結合測定法.
- TFIID複合体の形成の分析. TFIID複合体の形成の分析.
主要な成果:
- hTAFII250の遺伝子は,細胞サイクル進行に関与する遺伝子であるCCG1と同一である.
- 再結合されたhTAFII250はTBPに直接結合する.
- hTAFII250は,TFIID複合体の形成に参加しています.
結論:
- hTAFII250は,ヒトで初めて確認されたTAFである.
- この TAF は TBP と他の TAF との相互作用によって TFIID の組み立てに中心的な役割を果たす可能性があります.
- hTAFII250は,転写制御を細胞サイクルと結びつける.
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