ランダムな配列の大きなプールから新しいリボ酵素を分離した[注釈を参照]
1Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Science (New York, N.Y.)
|September 10, 1993
まとめ
科学者たちは,RNA分子に効率的に結合する新しい触媒RNA (リボ酵素) を発見しました. in vitroで進化したこれらのリボ酵素は,非触媒反応の何百万倍もの速度で結合速度を示します.
科学分野:
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
- RNAのカタリシス
背景:
- ランダムな配列から反復的インビトロ選択を用いた新しい触媒RNA (リボ酵素) の分離.
- タンパク質酵素RNA合成を模倣した5'-トリフォスファートに対する3'-ヒドロキシル攻撃によるRNA結合を触媒とするリボ酵素の特徴.
- また,非触媒反応は,3',5'-フォスフォディエステル結合を形成する.
関連する概念動画
02:47Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...