関連する実験動画
Updated: Jul 22, 2026
08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
ジャッゲッド: Notch1を活性化する哺乳類のリガンド
C E Lindsell1, C J Shawber, J Boulter
1Department of Biological Chemistry School of Medicine, University of California, Los Angeles 90024.
Cell
|March 24, 1995
まとめ
研究者らはネズミのcDNAクローン"ジャッジド"を特定し,脊椎動物の"ノッチ"のリガンドをコードしている. このジャッジド・ノッチの相互作用は,哺乳類の細胞を無差別状態に保つために極めて重要です.
科学分野:
- 発達生物学 発達生物学について
- 分子生物学は分子生物学である.
- 細胞シグナル伝達 細胞信号伝達
背景:
- Notchシグナル伝達経路は,多くの発達プロセスに不可欠な,高度に保存された細胞間の通信メカニズムです.
- Notch受容体を活性化する特定のリンガンドを理解することは,細胞の運命決定におけるその役割を明らかにするために重要です.
研究 の 目的:
- 脊椎動物のノッチ受容体の新しいリガンドを特定し,特徴づけること.
- 哺乳類の細胞分化におけるこのリガンドの機能的役割を調査する.
主な方法:
- ジャガードという名のネズミのcDNAクローンの分離とシーケンシング.
- ネズミの胚における遺伝子発現パターンを分析するために,in situ ハイブリダイゼーション.
- 細胞培養アッセイは,Jagged on Notch1シグナリングの生物学的活動を評価するものです.
主要な成果:
- ジャガドのcDNAは,無脊椎動物のノッチリンガンドに有意な同質性を持つタンパク質をコードする.
- ジャガドは,胚の発達中にノッチ1,ノッチ2,ノッチ3と異なる表現パターンが重なり合っている.
- Jaggedはネズミのノッチ1をミオブラストで活性化させ,筋肉細胞への分化を阻害する.
結論:
- Jaggedは,脊椎動物のノッチ受容体の機能リガンドである.
- ジャガードが例示したノッチ-リガンドの相互作用は,哺乳類の細胞を無差別状態に保ち,それによって発達中の細胞の運命決定に影響を与える役割を果たしています.
関連する概念動画
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:22Activation and Inactivation of G Proteins
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...