転写活性化器機能の選択的決定因子としての基礎プロモーター要素

G Das¹, C S Hinkley, W Herr

¹Cold Spring Harbor Laboratory, New York 11724, USA.

Nature

|April 13, 1995

まとめ

基礎プロモーター要素の配置は,転写因子が遺伝子発現を活性化させる方法を決定する. TATAボックスやPSEのような要素の特殊な配置は,アクティベータに対する異なる反応を可能にします.

科学分野:

分子生物学は分子生物学である.
遺伝子規制遺伝子規制
バイオケミストリーバイオケミストリー

背景:

ユカリオットの転写活性化には,アクティベーターと基礎転写因子が含まれています.
TATAボックスや近接配列要素 (PSE) などの基礎要素は,基礎転写複合体を構成する.
異なる基礎転写複合体が特定の活性化剤に対する異なる反応を媒介できるかどうかは不明である.

研究の目的:

基礎プロモーター要素の配置が転写活性化ドメインに対する反応に影響するかどうかを調査する.
基礎要素がアクティベータに差異的な反応を誘導できるかどうかを判断する.

主な方法:

人間のU6小核RNA (snRNA) プロモーターにおける基礎元素 (TATAボックスとPSE) の変異.
これらの変異がVP16とSp1の活性化ドメインに対する反応に与える影響を評価する.

主要な成果:

基礎要素の配置を変更すると,転写活性化ドメインに対する反応が変化します.
U6 snRNAプロモーターのTATAボックスまたはPSEの点変異は,VP16とSp1の活性化ドメインに対する反対の反応をもたらしました.
ベース要素は,活性化領域内の微妙な変化に対する感受性を示した.

結論:

基礎プロモーター要素の特定の配置は,転写の微分調節のためのメカニズムとして機能します.
基礎プロモーター要素は,異なるアクティベーターとそのドメインを区別する上で重要な役割を果たします.

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