ワキューラー移転に関与するグルタチオンS-トランスフェラーゼは,トウモロコシの遺伝子Bronze-2によってコーディングされています
K A Marrs1, M R Alfenito, A M Lloyd
1Department of Biological Sciences, Stanford University, California 94305-5020, USA.
Nature
|June 1, 1995
まとめ
トウモロコシのBronze-2遺伝子は,グルタチオンS-トランスファーゼ (GST) 酵素をコードする. この酵素は,アントシアニンをグルタチオンと結合させ,植物真空孔への輸送を促進します.
科学分野:
- 植物生化学 植物生化学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- グルタチオンS-トランスフェラーゼ (GSTs) は,グルタチオン結合体を形成することによって,ゼノバイオティクスを解毒します.
- 植物の真空は,特定のポンプを通じて,除草剤-グルタチオン結合体を含む様々な分子を封じ込めます.
- トウモロコシのBronze-2 (Bz2) 遺伝子は,アントシアニン生物合成と色素の堆積に不可欠です.
研究 の 目的:
- トウモロコシのブロンズ-2 (Bz2) 遺伝子産物の生化学的機能を決定するために.
- 植物真空中のアントシアニン結合におけるBz2の役割を調査する.
- 多様な植物分子の真空輸送のための潜在的な共通のメカニズムを探求する.
主な方法:
- GST活動を評価するために,トウモロコシ,アラビドopsis thaliana,およびEscherichia coliでBz2遺伝子を発現した.
- Bz2.2 を発現するトウモロコシ原生体におけるグルタチオンと結合したアントシアニン.
- アントシアニンの真空蓄積を研究するために,グルタチオンポンプ阻害剤であるバナダートを使用しました.
主要な成果:
- Bz2は,機能的なグルタチオンS-トランスファーゼ酵素をコードする.
- Bz2 を発現するトウモロコシ原生体からのアントシアニンは,グルタチオンと結合していることが判明しました.
- バナダート治療は,アントシアニンの真空蓄積を阻害し,グルタチオンポンプの役割を示しています.
結論:
- Bz2遺伝子産物は,アントシアニンの代謝に関与するグルタチオンS-トランスフェラーゼである.
- アントシアニンのグルタチオネーションは,トウモロコシの真空封じ込めに不可欠です.
- 植物は,バキュオールに多様な分子を隔離するために一般的なGST媒介メカニズムを使用する可能性があります.
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