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Updated: Aug 10, 2026
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Forebrain Electrophysiological Recording in Larval Zebrafish
Published on: January 24, 2013
神経伝達物質の放出におけるシナプス везикулの明確なプール
V A Pieribone1, O Shupliakov, L Brodin
1Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021, USA.
Nature
|June 8, 1995
まとめ
シナプシンが神経伝達物質の放出を調節するのは,シナプスを異なるプールに編成することによってである. シナプシン関連遠端プールは,高周波神経伝達を維持するために不可欠です.
科学分野:
- 神経科学は神経科学である.
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- 神経末端は,高度の膀の放出を示し,ユニークな分泌機能を持っています.
- シナプシンなどのニューロン固有のタンパク質は,神経伝達物質の放出を調節することに関与しています.
- シナプシンは,リン酸化によるシナプス水泡と細胞骨格の相互作用を調節すると考えられている.
研究 の 目的:
- シナプシンがシナプス池の組織化における役割を調査する.
- 神経伝達物質の放出におけるシナプシン関連膀の機能的重要性を決定する.
主な方法:
- 顕微鏡を用いた放出部位におけるシナプス胞池の特徴化.
- プールの動態を評価するためにシナプシン抗体のプレシナプス注射.
- 異なる刺激周波数下での神経伝達物質の放出の電気生理学的評価.
主要な成果:
- シナプシンの膀は,シナプシンを含んだディスタルプールとシナプシンを含まない近接プールに集まります.
- シナプシン抗体は,ディスタル膀プールを選択的に排除した.
- ディスタルプールの枯渇は,高周波 (18〜20 Hz) の神経伝達物質の放出を著しく低下させるが,低周波 (0.2 Hz) の放出を妨げない.
結論:
- シナプシンに関連した遠端の水泡池は,高周波ニューロン活動中に神経伝達物質の放出を維持するために不可欠です.
- シナプシンは,持続的な神経伝達に必要な,容易に放出できる膀のプールを維持する上で重要な役割を果たします.
関連する概念動画
01:12Synaptic Signaling
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
02:47The Synapse
Neurons communicate with one another by passing on their electrical signals to other neurons. A synapse is the location where two neurons meet to exchange signals. At the synapse, the neuron that sends the signal is called the presynaptic cell, while the neuron that receives the message is called the postsynaptic cell. Note that most neurons can be both presynaptic and postsynaptic, as they both transmit and receive information.
01:26Fusion of Secretory Vesicles with the Plasma Membrane
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
01:09Synaptic Signaling
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
01:26Chemical Synapses
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
01:26Chemical Synapses
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...