ニューレギュリンは神経筋シナプスに濃縮され,ACH受容体遺伝子発現を活性化します
S A Jo1, X Zhu, M A Marchionni
1Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115.
Nature
|January 12, 1995
まとめ
ニューレグリンは,筋肉細胞におけるアセチルコリン受容体 (AChR) 遺伝子発現を活性化させ,シナプス部位でのAChR遺伝子転写の信号であることを示唆しています. このシグナル伝達には,骨格筋のErbB2およびErbB3受容体が関与しています.
科学分野:
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- 骨格筋シナプスにおけるアセチルコリン受容体 (AChR) の局所化を調節する2つの経路があります.
- アグリンはACHRの再分配をシグナルし,ACHR遺伝子転写のための別の経路のシグナルはまだ不明である.
研究 の 目的:
- 神経ルギュリンがシナプス核におけるACHR遺伝子発現を活性化する信号であるかどうかを調査する.
- 骨格筋におけるニューレグルリン信号伝達のメカニズムを解明する.
主な方法:
- C2筋細胞における AChR 遺伝子発現に対するニューレグリンの効果を評価した.
- AChRデルタサブユニット遺伝子のニューレグルリン応答要素が特定されました.
- 抗体は,シナプス部位のニューレギュリンを局所するために使用されました.
- C2筋細胞におけるerbB2,erbB3,erbB4mRNAの発現を分析した.
- erbB2およびerbB3のニューレグルリン誘発型チロシンリン酸化を測定した.
主要な成果:
- ニューレグリンは,C2筋細胞のACHR遺伝子発現を活性化します.
- 神経ルギンの反応要素は,シナプス特異的な発現に責任のある181の塩基対の中にあります.
- ニューレギュリンはシナプス部位に濃縮され,神経や筋肉とは独立して持続します.
- C2筋細胞は,erbB2とerbB3を発現し,神経ルギンは,それらのチロシンリン酸化を刺激する.
結論:
- ニューレグリンは,シナプス部位でのACHR遺伝子転写を活性化する信号の候補である.
- 骨格筋における神経ルギンのシグナル伝達は,ErbB2/erbB3受容体複合体によって媒介されることがあります.
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