関連する実験動画

Updated: May 9, 2026

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
12:36

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme

Published on: October 20, 2010

E. coli tRNA前駆体のインビトロ加工

P Schedl, J Roberts, P Primakoff

    Cell
    |August 1, 1976
    PubMed
    まとめ

    この研究では,RNAase P変異体を使用してE. coliのtRNA前駆体処理を調査しました. 結果は,成熟したトランスファーRNA (tRNA) の形成には少なくとも3つの異なる酵素活動が関与していることを示しています.

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    Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...