プロモーター配列のプレベンディングは,TATA結合因子に対する親和性を高めます
J D Parvin1, R J McCormick, P A Sharp
1Center for Cancer Research, Massachusetts Institute for Technology, Cambridge 02139-4307.
Nature
|February 23, 1995
まとめ
TATA結合タンパク質 (TBP) は,DNAがメジャー・グリューブに向かって前向きに曲げられたときに,より高い親和度でDNAを結合します. このDNAトポロジーは,TBP結合に大きく影響し,転写制御に影響を与える可能性があります.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- TATA結合タンパク質 (TBP) は,転写の開始に不可欠です.
- TBPはDNAマイナー・グリューブのTATA要素を認識し,結合する.
- DNAの構造とトポロジーは,タンパク質とDNAの相互作用に影響を与える可能性があります.
研究 の 目的:
- TBP-TATA要素の結合親和性に対するDNAトポロジーの影響を調査する.
- DNAの曲げがTATA配列に対するTBPの親和に影響するかどうかを判断する.
主な方法:
- DNAトポロジーを制御するために,制約されたミニサークル戦略を利用した.
- TBPの結合親和性を線形,メジャー・グルーブ・ブント,マイナー・グルーブ・ブントDNAと比較した.
- TBP単独とホロ-TFIID転写複合体との結合が評価されています.
主要な成果:
- TBPは,線形DNAと比較して,メジャー・グリューブに向かってわずかに前向きに曲げられたDNAに対する100倍以上の親和性を示した.
- TBPは,メジャー・グルーブ・ボンドDNAとマイナー・グルーブ・ボンドDNAの300倍以上の親和性を示した.
- Holo-TFIIDは,DNAトポロジーに基づく同様の差別を示した.
結論:
- DNAのトポロジーは,特にメジャー・グリューブに向かって曲げられ,TBPの結合親和性を大幅に高めます.
- クロマチンなどの要因の影響を受けたDNAトポロジーの変化は,転写の調節に役割を果たす可能性があります.
- この研究は,転写因子の結合と制御におけるDNA適合の重要性を強調しています.
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