cAMP依存型タンパク質キナーゼとヘッジホッグは,ドロソフィラのイメージナルディスクにおけるデカペンタプレジック転写を調節する際に敵対的に作用する
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200.
Cell
|February 24, 1995
まとめ
cAMP依存タンパク質キナーゼ (PKA) は,ドロソフィラの発達におけるデカペンタプレジック (dpp) 発現を抑制する. PKAの活性喪失は子宮外DPPにつながり,細胞の運命とイメージナルディスクのパターン形成に影響を与えます.
科学分野:
- 発達生物学 発達生物学とは
- 分子遺伝学 分子遺伝学
- 細胞シグナリング 細胞シグナリング
背景:
- デカペンタプレジック (dpp) は,ドロソフィラのイメージナルディスクの発達に不可欠です.
- パターン形成には,dpp発現の正確な調節が不可欠である.
研究 の 目的:
- dpp発現の調節におけるcAMP依存タンパク質キナーゼ (PKA) の役割を調査する.
- PKAがドロソフィラのイメージナルディスクの細胞の運命とパターン形成にどのように影響するかを理解するために.
主な方法:
- ドロソフィラの遺伝子モザイクを使用した.
- 分析した細胞は,付属体や目の円盤にPKA活動が欠けている.
- 子宮の外部DPP発現と隣接する細胞への影響を評価した.
主要な成果:
- PKA活動が欠けている細胞は,眼の前部付属体と眼盤のコンパートメントで外視的にDPPを発現します.
- PKA欠乏細胞は,隣接する細胞の運命を影響し,前部パターンを変化させ,子宮外形変異遺伝的を誘発する.
- Pka変異細胞の組織化活動は,dpp に依存しています.
結論:
- PKAは,dpp表現を抑制するシグナル伝達経路の構成要素です.
- ヘッジホッグ (hh) 信号伝達は,この経路に敵対し,コンパートメントの境界や形態遺伝的でDPP発現を維持する.
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