フラゲラ・レギュロンの子宮外発現は,ボルデテラと宿主の相互作用の発展を変化させます

B J Akerley¹, P A Cotter, J F Miller

¹Department of Microbiology and Immunology, School of Medicine, University of California, Los Angeles 90024.

Cell

|February 24, 1995

まとめ

ボルデテラウイルス性制御 (bvgAS) は,遺伝子を活性化し抑制します. 運動性の遺伝子を抑制することは宿主コロニー化に不可欠であり,感染中に発現する遺伝子は細菌感染を妨げます.

科学分野:

微生物学微生物学とは
バクテリア病原菌の発生
遺伝子規制遺伝子規制

背景:

2つのコンポーネントのシステムは,細菌の適応と病原性にとって不可欠です.
Bordetella bvgASのロコスは,環境のシグナルの反応として,毒性の要因を調節する.
感染中のBvgAS媒介抑制の役割は不明である.

研究の目的:

Bordetella感染中にBvgAS抑制遺伝子の機能を調査する.
抑制された遺伝子が宿主植民に必要かどうかを判断する.
毒性の促進におけるBvgASの二重な役割を明らかにする.

主な方法:

ボルデテラの調節遺伝子の遺伝子操作.
異なる成長条件下での遺伝子発現パターンの分析.
ホストモデルにおけるバクテリアのコロニー化の評価.

主要な成果:

BvgASで抑制された運動性遺伝子は宿主コロニー化には必要ありません.
Bvg+フェーズ中の運動性遺伝子の発現は,気管の植民を損なう.
BvgASは,コロニー化因子を活性化し,抑制因子を抑制することによって,毒性を制御します.

結論:

BvgASを媒介する抑制は, Bordetellaの効果的な病原化に不可欠です.
非必須または有害な遺伝子を抑制すると,細菌のコロニー化が強化されます.
bvgASロクスは,感染を成功させるために複雑な規制ネットワークを編成します.

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