トマトの病気に対する耐性を与えるタンパク質キナーゼ遺伝子の地図ベースのクローニング
G B Martin1, S H Brommonschenkel, J Chunwongse
1Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY 14853-1902.
Science (New York, N.Y.)
|November 26, 1993
まとめ
トマトの植物は,Pseudomonas syringae pv.に対する耐性を獲得する. トマトは,Pto遺伝子を通して この遺伝子は,タンパク質キナーゼと似ており,植物防御信号経路で機能する可能性があります.
科学分野:
- 植物病理学 植物病理学
- 分子遺伝学 分子遺伝学
- 植物と微生物の相互作用
背景:
- トマトのPto遺伝子は,Pseudomonas syringae pv.の特定の株に対する耐性を提供しています. トマト トマト トマト トマト
- 病原性遺伝子のavrPtoを媒介する病原体種族は,この抵抗によって標的にされます.
研究 の 目的:
- トマトのPto媒介性耐性の原因となる遺伝子を特定し,特徴づけること.
- この植物の防御反応の基礎にある分子機構を明らかにする.
主な方法:
- 酵母人工染色体 (YAC) クローニングにより,Ptoゲノム領域を分離する.
- YACクローンを用いて葉の補完DNA (cDNA) ライブラリをスクリーニング.
- 候補遺伝子の共分離をPto.と確認するための遺伝子解析.
- 候補cDNAを持つ感受性の高いトマト植物の変容.
- 隔離された遺伝子のアミノ酸配列解析.
主要な成果:
- Ptoに関連した遺伝子ファミリーが特定され,少なくとも6人のメンバーがレジスタンスロカスと遺伝的に共存している.
- このファミリーのcDNAを持つ感受性の高い植物の変異は,病原体に対する耐性を授与しました.
- 推論されたアミノ酸配列は,セリン・スレオニンタンパク質キナーゼとの類似性を示しています.
結論:
- Pto遺伝子ファミリーは,Pseudomonas syringae pv.に対するトマトの防御に重要な役割を果たしています. トマト トマト トマト トマト
- Ptoは,病気に対する耐性のための植物信号伝導経路に関与するセリン・スレオニンタンパク質キナーゼとして機能する可能性が高い.
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