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パパの調節性DNA制御におけるメチル化パターンは,E. coliにおけるピエロネフライトに関連したピリ相変異である

B A Braaten¹, X Nou, L S Kaltenbach

¹Department of Pathology, University of Utah Health Sciences Center, Salt Lake City 84132.

|February 11, 1994

まとめ

DNAメチル化パターンは,E. coliにおけるピエロネフライトに関連したピリ (Pap) 発現を調節する. GATC1028でのメチル化は,Pap ON状態を抑制しますが,GATC1130でのメチル化は必要となります.

科学分野:

微生物学微生物学とは
エピジェネティクスエピジェネティクス
バクテリアの遺伝学

背景:

E. coliにおけるピエロネフライトに関連したピリ (Pap) 発現は,ONとOFF状態を切り替え,相変化を示す.
特定のGATC部位におけるDamメチルトランスフェラーゼによるDNAメチル化はこの相変化に影響する.

研究の目的:

GATC1028およびGATC1130におけるDNAメチル化が,Pap ON/OFF相変化の調節における特定の役割を調査する.
これらの部位でのメチル化が調節タンパク質結合と遺伝子発現に影響を与える分子機構を解明する.

主な方法:

メチル化を防ぐために,GATC1028とGATC1130のサイト指向型変異.
野生型および変異性E. coli菌株におけるパプフェーズ変異の分析.
タンパク質とDNAの相互作用を評価するための電泳運動シフトアッセイ (EMSA)

主要な成果:

GATC1028のメチル化は,LrpとPapiの結合を阻害することによって,ON状態を防ぐ.
GATC1130のメチル化はON状態に不可欠であり,ピリンプロモーターの近くでのLrp結合を阻害する可能性がある.
これらの部位の変異により,通常の相変化パターンが変化した.

結論:

特定のGATC部位でのDNAメチル化は,E. coliにおけるPap ON/OFFのスイッチングの重要な調節因子である.
GATC1028とGATC1130の微分メチル化により,調節性タンパク質相互作用の調節により相状態が決定されます.
Pap ONとOFF状態の間のメチル化主導の移行モデルが提案されています.

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