UltrabithoraxのDNA結合特異性は,別のホメオプロテインであるエクストラデンチクルとの協力相互作用によって調節されます
S K Chan1, L Jaffe, M Capovilla
1Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032.
Cell
|August 26, 1994
まとめ
ホメオティック遺伝子のUltrabithorax (Ubx) とAntennapedia (Antp) は,異なる身体パターンを指定しています. エクストラデンチクル (exd) タンパク質との協力的な相互作用は,Ubx DNAの結合を選択的に強化し,異なる発達的役割を説明します.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- ドロソフィラのホメオティック遺伝子であるウルトラビトラクス (Ubx) とアンテナペディア (Antp) は,セグメンタルパターンの発達に不可欠です.
- これらのタンパク質は,DNA結合の特異性を同等に in vitro 共有しているが, in vivo では異なる発達結果を決定する.
研究 の 目的:
- UbxとAntpの微分機能の基礎にある分子メカニズムを調査する.
- ホメオドメインタンパク質のDNA結合特異性を調節する他のタンパク質との協力相互作用の役割を調査する.
主な方法:
- タンパク質とDNAの相互作用を評価するためのインビトロDNA結合測定法.
- Ubx,Antp,およびエクストラデンチクル (exd) タンパク質間の協力結合の分析.
- Ubx と exd. の間の直接的なタンパク質-タンパク質相互作用の調査.
主要な成果:
- エクストラデンチクル (exd) タンパク質は,UbxのDNA結合親和性を選択的に強化するが,特定のDNA標的に対するAntpは強化しない.
- UbxとexdはDNAの隣接する部位に結合する.
- Ubxとexdの間の直接的な相互作用は,UbxのDNA結合状態を安定させます.
結論:
- exdのようなタンパク質との協力的な相互作用は,ホメオティックな遺伝子が明確なセグメンタルパターンを指定するために不可欠です.
- コファクターによるDNA結合特異性の選択的調節は,発達の遺伝子調節における重要なメカニズムである.
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