cAMP反応性元素結合タンパク質の標的型変異を有するマウスの長期的な記憶が欠けている
R Bourtchuladze1, B Frenguelli, J Blendy
1Cold Spring Harbor Laboratory, New York 11724.
Cell
|October 7, 1994
まとめ
CREB (cAMP反応性要素結合タンパク質) が欠けているマウスは,長期的な記憶が低下しているが,短期記憶は正常である. これは,CREB依存の転写が永続的な記憶の形成に不可欠であることを示唆しています.
科学分野:
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- cAMP反応性要素結合タンパク質 (CREB) は,長期記憶形成のためのタンパク質合成に関与しています.
- 長期増強 (Long-term potentiation,LTP) は,記憶の基礎となっていると考えられる細胞のメカニズムである.
研究 の 目的:
- 哺乳類の長期記憶におけるCREB同型 (アルファ,デルタ) の役割を調査する.
- 記憶プロセスとシナプス可塑性に対するCREB障害の影響を調べる.
主な方法:
- 恐怖コンディショニングと水迷路のタスクを用いたマウスでの行動研究.
- CREB変異マウスからの海馬のスライスにおけるLTPの電気生理学的記録.
主要な成果:
- CREBのアルファおよびデルタ同型が破壊されたマウスは,長期記憶に重大な欠陥を示した.
- これらの突然変異したマウスの短期記憶は影響を受けませんでした.
- CREBの変異した海馬のスライスにおけるLTPは,野生型の対照群とは異なり,急速に衰退した.
結論:
- CREB依存の転写は,哺乳類の長期記憶の強化に重要な役割を果たします.
- 特定のCREBアイソフォームは,メモリストレージの基礎となるシナプス可塑性を維持するために不可欠です.
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