関連する実験動画
Updated: Jun 19, 2026
08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
毛のないタンパク質の抑制剤は,割れ目受容体のシグナル伝達に参加します
M E Fortini1, S Artavanis-Tsakonas
1Howard Hughes Medical Institute, Department of Biology, Yale University, New Haven, Connecticut 06536.
Cell
|October 21, 1994
まとめ
NotchのシグナリングレギュレータであるSuppressor of Hairless (Su(H) の機能獲得変異が特定されました. クラッチシグナリングは,Su (H) タンパク質の局所化を制御し,ドロソフィラの発達における核イベントに影響を与えます.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- ノッチ・シグナリングは,発達中の細胞運命を決定する上で極めて重要です.
- 無毛の抑制剤 (Su(H)) は,ノッチ経路における重要な転写因子である.
- Su (H) 規制の理解は,発達過程の洞察を提供します.
研究 の 目的:
- ドロソフィラの眼の発達におけるNotchシグナル伝達に影響を与える突然変異を特定する.
- Su(H) タンパク質の活性を調節するメカニズムを調査する.
- 細胞の局所化におけるノッチとSu (H) の関係を解明する.
主な方法:
- ドロソフィラ・メラノガスターの遺伝子スクリーニングで,機能獲得アレルを分離する.
- ノッチ経路のコンポーネントとのアレル固有の遺伝子相互作用の分析.
- 細胞培養実験は,コエクスプレッションとリガンド結合アッセイを使用してタンパク質の局所化を研究します.
- 酵母相互作用トラップアッセイで,タンパク質とタンパク質の相互作用を確認します.
主要な成果:
- 脱毛サプレッサー (Su(H)) の単離された新しい機能獲得アレル.
- Su(H),Notch,Delta,deltex,およびマスターマインドとの間のアレル固有の相互作用が実証されています.
- Notchシグナリングは,細胞質と核の間のSu (H) タンパク質の局所化を調節することを示した.
- 細胞内Notch cdc10/ankyrinの繰り返しは,Su (H) の細胞質保持に重要なものであることを確認した.
結論:
- ノッチ・シグナリングは,Su(H) DNA結合タンパク質の細胞の位置と活性を直接制御する.
- このメカニズムは,細胞表面受容体の活性化が核転写にどのように影響するかを強調しています.
- 発見は,発達中の遺伝子発現のノッチ媒介調節のための分子基礎を提供します.
さらに関連する動画
関連する概念動画
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...