関連する実験動画
Updated: Jun 27, 2026

07:44
High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
RNAポリメラーゼIによる転写終結のためのモデル
1Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.
Cell
|November 4, 1994
まとめ
酵母RNAポリメラーゼIの終結には,Reb1p結合と5'DNAが必要です. Reb1p結合は一時停止を引き起こし,隣接するDNAは放出を助長し,一般的な真核終結モデルを形成する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- バイオケミストリー バイオケミストリー
背景:
- トランスクリプションの終結は,遺伝子調節において極めて重要です.
- ユカリオットRNAポリメラーゼは,特定のDNA配列とタンパク質因子を終了のために必要とします.
- イーストRNAポリメラーゼI (Pol I) 終結メカニズムは完全に理解されていません.
研究 の 目的:
- イーストRNAポリメラーゼIの転写終止の基礎となる分子メカニズムを解明する.
- Reb1p結合部位の機能的役割とその5'端末の側面配列を特定する.
- ユカリオットにおける転写終結の一般的なモデルを提案する.
主な方法:
- イーストRNAポリメラーゼIの転写終結部位の分析.
- Reb1p結合部位と隣接DNA配列の役割を調査する.
- 終端要素のポリメラーゼ特異性を特徴づける.
主要な成果:
- イーストポルIの終結には11bpのReb1p結合部位と約46bpの5'横のDNAが必要です.
- Reb1p結合は,ポリメラーゼ特異性とは無関係に,パウズ要素として作用する.
- 5'側面配列は,ポリメラーゼ特異的な放出要素として機能する.
結論:
- 転写終結は,ポリメラーゼ特異的な放出要素の文脈内の非特異的な信号によって誘発されたポリメラーゼの停止を含むモデルが提案されています.
- このモデルは,真核細胞の転写終結を理解するための一般的な枠組みを提供します.
- この研究は,トランスクリプションの終結を調節する際に,DNA結合タンパク質とDNAの側面の異なる役割を強調しています.
関連する概念動画
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
Transcription Attenuation in Prokaryotes
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Bacterial Transcription
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...

