ドロソフィラ胚におけるオトクリン・パラクリン・シグナル伝達のための異なる経路 翼のないシグナル伝達
1Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, Denver 80262.
Nature
|December 1, 1994
まとめ
ドロソフィラ胚の無翼 (Wg) とヘッジホッグ (Hh) 信号は相互依存しています. この研究は,このループを分離し,隣接する細胞へのWg信号伝達と対照的に,直接的なWg自己調節のための明確な遺伝的要件を明らかにします.
科学分野:
- 発達生物学 発達生物学について
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- Wingless (Wg) とHedgehog (Hh) は,ドロソフィラ胚の細胞運命を決定するために重要な分泌タンパク質です.
- これらのシグナル伝達経路は相互依存を示し,WgはHhの表現を安定させ,HhはWgの表現を安定させます.
- このポジティブなフィードバックループは,胚の発達に不可欠ですが,直接的なWg自己調節を覆います.
研究 の 目的:
- 遺伝子的に Wg-Hh 陽性フィードバックループを分離するには,zeste-white3 (zw3) とpatched (ptc) 変異体を使用します.
- オトクリンWgシグナル伝達とパラクリンWgシグナル伝達との比較において,異なる遺伝的成分を調査する.
- 早期のHh依存の段階と後の発達段階におけるWg自己調節の時間的差異を分析する.
主な方法:
- ドロソフィラ胚におけるゼステ・ホワイト3 (zw3) とパッチされた (ptc) 変異背景を利用した.
- 遺伝子操作を用いて,自己決定性無翼信号を分離し,研究した.
- 異なるシグナル条件下でのWg自己調節のための遺伝的要件を比較した.
主要な成果:
- 直接的なWg自己調節は,zw3やアーマディロ (腕) と比較して,fused (fu),smo (smo),cubitus interruptus (ci) などの遺伝子に対する差異的な依存を示しています.
- 初期のHh依存期におけるWg自己調節は,後の自己調節とは異なる.
- グースベリー (GSB) は,後の段階とは異なり,初期のWg自己調節には参加しません.
結論:
- オトクリンWgシグナル伝達には,パラクリン機能とは異なるユニークな遺伝的基盤があります.
- Wg自己調節の遺伝的コントロールは,胚の発達中に進化する.
- これらの異なる規制メカニズムの理解は,発達シグナリングの強度についての洞察を提供します.
関連する概念動画
Autocrine Signaling
Autocrine signaling is one of the many signaling mechanisms that function inside multicellular organisms to carry out intercellular communication. In this type of signaling mechanism, the same cell that secretes an extracellular signaling molecule also expresses the receptors to bind and respond to that signaling molecule.
Autocrine Signaling in Macrophages
Under normal physiological conditions, autocrine signaling is essential for maintaining homeostasis. This process is well characterized in...
Autocrine Signaling in Macrophages
Under normal physiological conditions, autocrine signaling is essential for maintaining homeostasis. This process is well characterized in...
Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...


