関連する実験動画
Updated: Jun 26, 2026

11:00
Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
Published on: May 17, 2010
クセノプス・コーディン (Xenopus chordin):オーガナイザー特異のホメオボックス遺伝子によって活性化される新しいドーサライジング因子
Y Sasai1, B Lu, H Steinbeisser
1Molecular Biology Institute, University of California, Los Angeles 90024-1737.
Cell
|December 2, 1994
まとめ
研究者らは,胚の発達における重要な要因であるクセノプスのコルディン遺伝子を特定した. コルディンは強力なドルザライジング因子として作用し,頭,幹,尾の形成を調節するために不可欠です.
科学分野:
- 発達生物学 発達生物学とは
- 分子生物学は分子生物学である.
- Xenopus laevis 研究研究について
背景:
- GoosecoidやXnot2のようなオーガナイザー固有のホメオボックス遺伝子は,胚のパターニングにおいて重要な役割を果たします.
- 軸向的な発達を調節する分子機構を理解することは,発達生物学にとって根本的なものです.
研究 の 目的:
- Xenopus.の胚軸形成に関与する新種の遺伝子を分離し,特徴づけること.
- ドルサライゼーションと軸向の発達を調節する新発見のコルディン遺伝子の役割を調査する.
主な方法:
- 差別スクリーニングは,オーガナイザー固有のホメオボックス遺伝子によって活性化された遺伝子を識別するために使用されました.
- コルディン遺伝子発現パターンは,胚の発達中に分析されました.
- コルディンmRNAのマイクロインジェクションは,腹腔内胚の軸向発達への影響を評価するために実施されました.
主要な成果:
- 新型遺伝子であるchordinが分離され,信号配列と4つのCys豊富なドメインを持つタンパク質をコードした.
- コルディン発現は,グースコイドの後にスペーマンのオーガナイザーで開始され,アクティビンの誘導のために新しいタンパク質合成を必要とします.
- コーディンmRNAのマイクロインジェクションは,成功裏に双子の軸を誘導し,腹部化したXenopus胚の軸的発達を救出しました.
結論:
- コルディンは,胚の組織センターにおける細胞-細胞の相互作用を調節するために重要な強力なドルザライジング因子です.
- コーディンの時間的・空間的な表現は,頭,体,尾の発達における重要な役割を示唆しています.
- 胎内胚を救出するコルディンの能力は,主体軸の確立におけるその重要性を強調しています.
関連する概念動画
X-Inactivation
The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
Combinatorial Gene Control
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
The Ratio of X Chromosome to Autosomes
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Dosage Compensation
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...
Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...

