脂質活性化転写因子であるPPARガンマ2によるフィブロブラストにおけるアディポゲネシスの刺激
P Tontonoz1, E Hu, B M Spiegelman
1Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|December 30, 1994
まとめ
ペロキシソーム増殖器活性化受容体ガンマ2 (PPARガンマ2) は脂肪細胞の発達を誘発する. この核受容体は,脂質代謝を脂肪細胞の分化と結びつけ,脂肪組織の調節に極めて重要です.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- エンドクリノロジー エンドクリノロジー
背景:
- ペロキシソーム増殖器活性化受容体ガンマ2 (PPARガンマ2) は,アディポサイト特異の核ホルモン受容体である.
- PPARガンマ2は,主要な脂肪細胞増強剤を調節し,その活動は多不飽和脂肪酸を含む脂質によって調節されます.
研究 の 目的:
- 脂肪細胞の分化におけるPPARガンマ2の役割を調査する.
- アディポゲネシスの他の転写因子とのPPARガンマ2の協力効果を探求する.
- PPARガンマ2と脂質代謝,脂肪組織発達の関連性を理解する.
主な方法:
- PPARガンマ2のレトロウイルスの発現は,培養した線維芽細胞で.
- PPAR活性化剤による治療は,差異化に対する用量依存的効果を評価する.
- C/EBPアルファとの共同発現の研究で,シネージ効果を評価する.
主要な成果:
- レトロウイルスPPARのガンマ2発現は,線維芽細胞の脂肪分化を刺激した.
- PPAR活性化剤は,PPARガンマ2発現細胞の分化を促進し,投与量に依存した方法で作用した.
- C/EBPアルファはPPARガンマ2と連携して,脂肪細胞の分化プログラムを大幅に強化した.
結論:
- PPARガンマ2は,脂肪系の開発を調節する重要な生理学的役割を果たします.
- 内生性脂質活性化剤は,PPARガンマ2活性を調節し,脂肪細胞の分化と全身性脂質代謝を結びつける.
- PPARガンマ2とC/EBPアルファは,アディポゲネシスを誘発するために,連携して作用する.
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