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Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These antibiotics are selectively...

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関連する実験動画

Updated: Jul 15, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

SeqA:E. coliにおけるレプリケーション開始の負の調節剤.

M Lu1, J L Campbell, E Boye

  • 1Department of Biochemistry and Molecular Biology Harvard University, Cambridge, Massachusetts 02138.

Cell
|May 6, 1994
PubMed
まとめ

SeqA遺伝子は,早発を防ぐことによって,細菌のDNA複製の開始を制御する. この遺伝子は負の調節体として作用し,細胞サイクルを正確に制御するためのポジティブな要因をバランスとします.

科学分野:

  • 分子生物学は分子生物学である.
  • 微生物学 微生物学とは
  • バクテリアの細胞サイクル規制

背景:

  • 細菌のDNA複製は,単一の起源,ORICで始まる.
  • 急速に成長する細胞では,複数の oriC コピーは存在し,細胞周期ごとに1回同期的に発動します.
  • シーケストレーションプロセスは,新たに複製された起源のヘミメチル化されたGATCサイトをターゲットにすることで,二次イニシアチブを防止します.

研究 の 目的:

  • E. coli. の封じ込めプロセスに責任を負う遺伝子を特定する.
  • DNA複製の開始を調節する特定された遺伝子の役割を調査する.
  • 複製制御におけるポジティブとネガティブな規制要素のバランスを理解する.

主な方法:

  • 隔離に必要な遺伝子を特定するための遺伝子スクリーニング.
  • 複製の開始における同定遺伝子 (seqA) の機能を評価するためのインビボアッセイ.
  • seqAがヘミメチル化原産物と初期因子との相互作用の分析.

主要な成果:

  • ヘミメチル化起源のシーケストレーションに不可欠な遺伝子seqAの識別.
  • seqAがプライマリレプリケーション開始の負の変調子として作用することを実証.

さらに関連する動画

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

関連する実験動画

Last Updated: Jul 15, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

  • seqAの機能が,以前に特定された陽性開始因子と対照的であるという観察.

    • 結論:

    • バクテリアのDNA複製の開始を正確に制御するには,陽性と陰性の規制要素のバランスが求められます.
    • SeqAタンパク質はネガティブレギュレータとして重要な役割を果たし,早すぎる複製を防ぐ.
    • SeqAは,レプリケーション開始コンポーネント間の相互作用を媒介する協力性の要因として機能する可能性があります.