関連する実験動画
Updated: May 13, 2026

07:37
CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
Published on: June 25, 2017
トランスクリプション延長における非トランスクリプションDNA鎖部位の機能
1Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.
Cell
|July 29, 1994
まとめ
ファグのラムダ転写の一時停止は,QアンチターミネーターによるRNAポリメラーゼの改変に不可欠である. この一時停止に影響する突然変異は,転写されていないDNA鎖を明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- ウイルス学 ウイルス学 ウイルス学
背景:
- ファグ・ラムダ・ラット・ゲンのオペロンは,ウイルスの複製のための正確な規制を必要とします.
- トランスクリプションアンチターミネータータンパク質Qは,RNAポリメラーゼを改変し,早期終結イベントを克服するために不可欠です.
研究 の 目的:
- ファグのラムダQタンパク質によるRNAポリメラーゼ変異における特定の転写停止の役割を調査する.
- この調節的な休止を媒介するDNA要素を解明する.
主な方法:
- ファグ・ラムダのサイト誘導性変異が遅い遺伝子オペロンである.
- ヘテロデュプレックスDNAテンプレートを使用したインビトロ転写アッセイ.
- RNAポリメラーゼの停止と改変の分析.
主要な成果:
- オペロンの+16/+17位置での長期間の休止は,Qタンパク質媒介RNAポリメラーゼの改変に不可欠である.
- 転写バブル内の変異 (例えば+2,+6) は,一時停止とQタンパク質の修正を廃止する.
- これらの重要な部位は,非転写DNA鎖を通して機能します.
結論:
- 転写されていないDNA鎖は,転写の延長を調節する上で,これまで認識されていない重要な役割を果たしています.
- トランスクリプションの停止は,DNA配列とタンパク質の相互作用によって調節される重要な規制メカニズムです.
関連する概念動画
Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
DNA-only Transposons
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...

