Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Mass Spectrometers01:16

Mass Spectrometers

9.1K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
9.1K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.1K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.1K
IR Spectrometers01:25

IR Spectrometers

2.7K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
2.7K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.6K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.6K
Chemistry of the Cell02:58

Chemistry of the Cell

48.4K
The cell is chemically composed of water, organic molecules and inorganic ions.
Water
The polarity of the water molecule and its resulting hydrogen bonding makes water a unique substance with special properties that are intimately tied to the processes of life. Life originally evolved in an aqueous environment, and most of an organism’s cellular chemistry and metabolism occur inside the aqueous contents of the cell’s cytoplasm. Special properties of water are its high heat capacity...
48.4K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.2K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2.2K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

11beta-hydroxysteroid dehydrogenases are regulated during the pulmonary granulomatous response to the mycobacterial glycolipid trehalose-6,6'-dimycolate.

Neuroimmunomodulation·2009
Same author

Requisite role for complement C5 and the C5a receptor in granulomatous response to mycobacterial glycolipid trehalose 6,6'-dimycolate.

Scandinavian journal of immunology·2005
Same author

Dysregulated response to mycobacterial cord factor trehalose-6,6'-dimycolate in CD1D-/- mice.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research·2002
Same author

A role for complement C5 in organism containment and granulomatous response during murine tuberculosis.

Scandinavian journal of immunology·2001
Same author

Bioavailability of tobramycin after oral delivery in FVB mice using CRL-1605 copolymer, an inhibitor of P-glycoprotein.

Life sciences·2000
Same author

Cytokine message and protein expression during lung granuloma formation and resolution induced by the mycobacterial cord factor trehalose-6,6'-dimycolate.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research·2000

関連する実験動画

Updated: Feb 12, 2026

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.3K

タンパク質化学のための高解像度質量スペクトロメーター

Y Li1, R L Hunter, R T McIver

  • 1Department of Chemistry, University of California, Irvine 92717.

Nature
|August 4, 1994
PubMed
まとめ

マトリックスアシストレーザー脱吸収/イオン化 (MALDI) は,ペプチドとタンパク質の分子量を正確に測定します. フーリエ変換質量スペクトロメトリ (FTMS) は, MALDI分析の従来の飛行時間の方法よりも感度と質量の精度が向上しています.

科学分野:

  • アナリティカル・ケミストリー (Analytical Chemistry) とは
  • バイオケミストリー バイオケミストリー
  • スペクトロメトリースペクトロメトリー

背景:

  • マトリックスアシストレーザー脱吸収/イオン化 (MALDI) は,バイオ分子を分析するために広く使用されている技術です.
  • Time-of-flight mass spectrometry (TOF MS) は MALDI と組み合わせた従来の検出方法である.
  • 複雑なサンプルに対するTOF MSの感度および質量測定の精度には制限があります.

研究 の 目的:

  • MALDIの代替検出方法としてフーリエ変換質量スペクトロメトリ (FTMS) を評価する.
  • FTMSとTOF MSの性能を,感度と質量の精度において比較する.
  • 高精度分子量決定のためのFTMSの利点を強調する.

主な方法:

  • MALDIはペプチドとタンパク質をイオン化するために使用されました.
  • レーザーで生成されたイオンは,飛行時間質量スペクトロメトリー (TOF MS) とフーリエ変換質量スペクトロメトリー (FTMS) を使用して検出されました.
  • 質量測定の精度と感度が,両方の検出技術で評価されました.

主要な成果:

  • FTMSは,TOF MSと比較して,かなり高い質量測定精度を示した.

さらに関連する動画

Combustion Chemistry of Fuels: Quantitative Speciation Data Obtained from an Atmospheric High-temperature Flow Reactor with Coupled Molecular-beam Mass Spectrometer
07:24

Combustion Chemistry of Fuels: Quantitative Speciation Data Obtained from an Atmospheric High-temperature Flow Reactor with Coupled Molecular-beam Mass Spectrometer

Published on: February 19, 2018

10.5K
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
09:14

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Published on: July 20, 2016

40.7K

関連する実験動画

Last Updated: Feb 12, 2026

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
08:23

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Published on: March 9, 2018

9.3K
Combustion Chemistry of Fuels: Quantitative Speciation Data Obtained from an Atmospheric High-temperature Flow Reactor with Coupled Molecular-beam Mass Spectrometer
07:24

Combustion Chemistry of Fuels: Quantitative Speciation Data Obtained from an Atmospheric High-temperature Flow Reactor with Coupled Molecular-beam Mass Spectrometer

Published on: February 19, 2018

10.5K
Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
09:14

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Published on: July 20, 2016

40.7K
  • レーザーで生成されたイオンを検出するFTMSの感度が優れていることが判明しました.
  • FTMSと組み合わせたMALDIは,分子量分析のための強化された能力を提供します.

    • 結論:

    • フーリエ変換質量スペクトロメトリは,MALDIベースの分子量決定の重要な進歩を表しています.
    • FTMSは感度と精度を向上させ,従来のTOF MSを上回る.
    • この技術は,ペプチドとタンパク質の正確な分析に大きな希望を持っています.