新しいサイクリンがMO15/CDK7と結合し,CDK活性化キナーゼを形成する
1Department of Physiology, University of California, San Francisco 94143-0444.
Cell
|August 26, 1994
まとめ
哺乳類のCDK活性化キナーゼ (CAK) は,MO15とサイクリンHで構成されています.このCDK7-サイクリンH複合体は,細胞周期進行に不可欠な他のサイクリン依存キナーゼ (CDK) を活性化します.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- サイクリン依存キナーゼ (CDK) は細胞サイクルを調節する.
- CDKsの活性化には,CDK活性化キナーゼ (CAK) によるリン酸化が必要です.
- 哺乳類のCAKの構成は,以前は特徴づけられていなかった.
研究 の 目的:
- 哺乳類のCAK酵素を精製し,特徴づけること.
- 哺乳類CAK.を含むサブユニットを識別する.
- 浄化されたCAKホロ酵素の機能を決定する.
主な方法:
- 哺乳類の細胞からCAKの浄化.
- タンパク質の配列決定とcDNAクローニングにより,サブユニットを特定する.
- インビトロ溶解とキナーゼアッセイ.
主要な成果:
- CAKは2つのサブユニット複合体 (42 kDaと37 kDa) として浄化されます.
- 42 kDa のサブユニットはMO15 ホモログであり,37 kDa のサブユニットは新型サイクリンHである.
- 再構成されたCAKはCDK7-サイクリンHで,CDK2およびCDC2-サイクリン複合体を活性化します.
- MO15/CDK7に保存されたスレオニンは,CAKの活性に不可欠です.
結論:
- 哺乳類のCAKはCDK-サイクリン複合体であり,CDK7-サイクリンHとして識別されています.
- CDK7-サイクリンHは,他のCDKをリン酸化し活性化させ,細胞サイクル制御に重要な役割を果たします.
- この発見は,CDK活性化と細胞周期調節の分子機構を明らかにする.
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02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
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