Shigella flexneriが上皮細胞間で広がるためには,カデリン発現が必要である

P J Sansonetti¹, J Mounier, M C Prévost

¹Unité de Pathogénie Microbienne Moléculaire Institut National de la Santé et de la Recherche Médicale Unité 389, Institut Pasteur, Paris, France.

Cell

|March 11, 1994

まとめ

Shigella flexneriバクテリアは,宿主細胞の機械を乗っ取ることで,細胞間で広がります. カデリン発現は,この細菌の細胞から細胞への侵入プロセスに不可欠です.

科学分野:

微生物学微生物学とは
細胞生物学細胞生物学
パトジェネシス (病原生)

背景:

Shigella flexneriは,ヒトの大腸に感染するグラム陰性細菌です.
細胞内移動と細胞間拡散は,S. flexneri. の主要な毒性メカニズムです.

研究の目的:

S.flexneriが細胞から細胞に拡散するために利用する宿主細胞の成分を調査する.
細胞粘着分子 (CAMs) が表皮細胞間のバクテリアの侵入を媒介する役割を決定する.

主な方法:

親型,L-CAMトランスフェクト型 (S180L),N-カデリントランスフェクト型 (S180cadN) を含むS180マウス線維芽細胞肉腫細胞系を使用した.
これらの異なる細胞系モデルにおいて,S.flexneriの細胞間拡散の過程を観察し,分析した.

主要な成果:

S.flexneriは宿主細胞のアクチンとアクチン結合タンパク質を乗っ取り,細胞内移動のためのモーターを作成します.
バクテリアの細胞から細胞への通過は,突起の形成と中間接合部材の利用を含む.
カデリン,特にN-カデリンの発現は,S.flexneriの細胞間拡散に不可欠であることが示されました.

結論:

カデリン媒介の粘着は,シゲッラ・フレクスネリが宿主細胞から別の宿主細胞に広がるのに不可欠です.
これらのバクテリアの侵入メカニズムを理解することで,シゲラ菌感染症に対する治療戦略を策定することができます.

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