関連する実験動画
Updated: May 7, 2026
06:17
Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
膀性口腔炎ウイルスのグリコプロテインは,エンドプラズマ網膜から輸出中に分類され,濃縮されます
W E Balch1, J M McCaffery, H Plutner
1Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
Cell
|March 11, 1994
まとめ
新しく合成されたタンパク質は,エンドプラズマ網膜 (ER) 輸出の際に膀に分類され,濃縮され,大量フローモデルに異議を唱えます. このER媒介による分類は,タンパク質が分泌経路に効率的に入り込むことを保証します.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- プロテインの密輸,人身売買
背景:
- タンパク質は,内プラズマ網膜 (ER) から,膀輸送経由でゴルギ器官に運ばれます.
- 支配的なモデルは,タンパク質の分類がトランス・ゴルギ・ネットワーク (TGN) のみで行われ,ER-to-Golgiの輸送はバルクフローに依存することを示唆している.
研究 の 目的:
- ER輸出中のタンパク質の分類と濃縮のメカニズムを調査する.
- タンパク質の分類がゴルギ装置の前に起こるかどうかを判断する.
主な方法:
- 定量免疫電子顕微鏡を用いて,ウイルスのタンパク質の移動と濃度を追跡した.
- 膀性口腔炎ウイルスグリコタンパク質 (VSV-G) は,ERの輸出動態を研究するためのモデルタンパク質として使用されました.
主要な成果:
- VSV-Gは,ERから膀の芽生えの間に,ERの定住タンパク質から分類されることが観察されました.
- ER出所中に40〜80nmの膀にVSV-Gの5〜10倍濃度が検出されました.
- ゴルギ前ベジキュラキャリアにおけるこの濃度は,VSV-G輸送の主要な濃縮段階を表しています.
結論:
- ERの輸出は,選択的なプロセスを示すバルクフローによってのみ媒介されるものではありません.
- ERはタンパク質を積極的に分類し,濃縮し,分泌経路への効率的な入り口を確保します.
- この研究は,タンパク質の輸出に対するER制御の予期せぬレベルを明らかにしています.
関連する概念動画
01:49Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
01:38Intralumenal Vesicles and Multivesicular Bodies
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
01:45Overview of Protein Sorting and Transport
Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation. In gated transport, folded...
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation. In gated transport, folded...
00:59COP Coated Vesicles
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of different...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:32Export of Misfolded Proteins out of the ER
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...