植物DNAウイルスの2つのタンパク質は,核とプラズモデスマル輸送を調整する
A O Noueiry1, W J Lucas, R L Gilbertson
1Department of Plant Pathology, University of California, Davis 95616.
Cell
|March 11, 1994
まとめ
ビーン・ダワーフ・モザイク・ジェミニウイルスの移動タンパク質BR1とBL1は,ウイルスのDNA輸送を促進する. BR1は核の輸出を介し,BL1はプラズモデスマタを通じて細胞間移動を促進する.
科学分野:
- 植物ウイルス学 植物ウイルス学
- 分子植物病理学 分子植物病理学
- 細胞生物学 細胞生物学
背景:
- 植物ウイルスは,プラズモデスマタを介して系統的に広がります.
- ウイルスの細胞間移動のメカニズムは完全に理解されていません.
研究 の 目的:
- ビーン・ダワーフ・モザイク・ジェミニウイルス (単一鎖DNAウイルス) の2つの運動関連タンパク質 (BR1とBL1) がウイルス輸送における役割を調査する.
- 核の輸出と細胞から細胞への移動におけるタンパク質の機能を明らかにする.
主な方法:
- 発現したウイルスタンパク質のインビオ機能分析.
- E. coli のタンパク質発現は
- 植物細胞へのマイクロインジェクション.
主要な成果:
- BL1タンパク質は,細胞から細胞への広範な移動を促進します.
- BL1は,メソフィール細胞におけるプラズモデスマルサイズの排除限界を増加させる.
- BL1は,二重鎖DNAの細胞間移動を促進する.
- BR1タンパク質は,単一および二重鎖のウイルスDNAの両方の核からの移動を媒介する.
結論:
- BR1とBL1のタンパク質は,ウイルスのDNA輸送において,異なるが協調的な役割を果たしている.
- BR1とBL1は,ウイルスのDNAが核およびプラズモデスマル障壁を越えて移動することを促進する.
- 植物における細胞間マクロ分子輸送の直接的な証拠を提供する.
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