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Updated: May 13, 2026
08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
JNK1:紫外線とHa-Rasによって刺激されるタンパク質キナーゼで,c-Jun活性化ドメインを結合してリン酸化する
1Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01605.
Cell
|March 25, 1994
まとめ
紫外線はAP-1とNF-カッパBを含む細胞反応を誘発する. 研究者らは,UVとオンコタンパク質によって活性化される新しいキナーゼであるJNK1を特定し,c-Junをリン酸化し,腫瘍増殖に潜在的に寄与している.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- 哺乳類の細胞は,AP-1とNF-kappa B.によって媒介される,紫外線被曝時に急速な遺伝子発現の増加を示します.
- AP-1のトランスクリプション活動は,c-Jun NH2-端末活性化ドメインのリン酸化の増加によって強化されます.
研究 の 目的:
- 紫外線反応に関与する新しいキナーゼであるJNK1をクローンし,特徴づけること.
- 紫外線照射とオンコタンパク質によって活性化された信号伝達経路におけるJNK1の役割を調査する.
主な方法:
- JNK1遺伝子の分子クローニングと特徴付け.
- 紫外線照射とHa-RasによるJNK1活性化の評価.
- c-JunトランザクティベーションドメインのJNK1結合とリン酸化を分析する.
主要な成果:
- MAPキナーゼの親戚であるJNK1は,UV反応中に二重リン酸化によって活性化されます.
- Ha-RasはJNK1を部分的に活性化し,UV誘発活性化を強化します.
- JNK1は,セル-63とセル-73でc-Junをリン酸化し,トランザクティベーション領域に結合する.
結論:
- JNK1は,UVとオンコタンパク質によって活性化される新しい信号伝導経路の重要な構成要素です.
- JNK1の活性化は,紫外線照射に対する細胞応答に関与しており,腫瘍増殖に作用する可能性がある.
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