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Updated: Aug 5, 2026

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

活性化欠陥のTBP変異が酵母における転写開始に及ぼす影響

T K Kim1, S Hashimoto, R J Kelleher

  • 1Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.

Nature
|May 19, 1994
PubMed
まとめ

研究者らは,基礎活性に影響を与えることなく,活性化剤依存転写をブロックする酵母TATA結合ポリペプチド (TBP) 変異を特定しました. これは,GAL4-VP16のようなアクティベーターが,TBPとTFIIBと相互作用して,転写開始を調節する方法を示しています.

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