JNKは,Tリンパ球の共刺激中に信号統合に関与しています
1Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093-0636.
Cell
|June 3, 1994
まとめ
T細胞の活性化には複数の信号が必要です. JNK1/JNK2キナーゼは,T細胞受容体 (TCR) とCD28共刺激からの信号を統合して,インタールイキン-2 (IL-2) の生成に不可欠です.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 細胞シグナリング 細胞シグナリング
背景:
- Tリンパ球の活性化とインタールイキン-2 (IL-2) の生成には,異なるシグナル伝達経路が必要である.
- 重要な信号は,ホルボールエステル (TPA) とCa2+イオノフォール,またはT細胞受容体 (TCR) とCD28共刺激を含む.
研究 の 目的:
- ミトゲン活性化タンパク質 (MAP) キナーゼに対する異なるT細胞活性化刺激の影響を調査する.
- T細胞活性化とIL-2生成のための信号を統合する特定のMAPキナーズの役割を明らかにする.
主な方法:
- TPA/Ca2+イオノフォアとTCR/CD28の共刺激を用いたT細胞の刺激.
- ウェスタン・ブロッティングまたはキナーゼアッセイを用いたJNK1/JNK2およびERK1/ERK2活性化の評価.
- シクロスポリンA (CsA) とJNK固有の阻害剤を用いた抑制研究.
主要な成果:
- JNK1とJNK2の完全な活性化には,個々の信号ではなく,組み合わせた刺激 (TPA/Ca2+またはTCR/CD28) が必要です.
- サイクロスポリンA (CsA) は,IL-2誘導に対するその効果を反映して,JNKの協同活性化を阻害しました.
- ERK1とERK2は,TPAまたはTCR刺激だけで活性化され,Ca2+,CD28またはCSAの影響を受けませんでした.
- JNK媒介によるユン・リン酸化の抑制により,IL-2プロモーターの活性が低下した.
結論:
- T細胞活性化のための信号統合は,JNK活性化レベルで行われます.
- JNKキナーゼは,TCRとCD28の共刺激の後方に IL-2の生成を媒介する上で重要な役割を果たします.
関連する概念動画
NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Interactions Between Signaling Pathways
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
NF-kB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...


