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インターフェロンによって誘発されるLDL受容体の抗ウイルス溶解形態

D G Fischer¹, N Tal, D Novick

¹Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.

Science (New York, N.Y.)

|October 8, 1993

まとめ

インターフェロンは,溶解性低密度リポプロテイン受容体 (LDLR) を誘導し,膀性口炎ウイルス (VSV) 感染症と闘います. この溶解性LDLRタンパク質は,ウイルス結合ではなく,ウイルスの集合を妨害することによってVSVを抑制します.

科学分野:

ウイルス学ウイルス学ウイルス学
免疫学免疫学とは
分子生物学は分子生物学である.

背景:

インターフェロン (IFN) は,ウイルス感染症に対する先天的な免疫反応を誘発する重要なサイトカインです.
IFNは,細胞内抗ウイルスタンパク質を誘導し,また,抗ウイルス特性を持つ細胞外溶性タンパク質を誘導します.

研究の目的:

膀性口炎ウイルス (VSV) 感染を阻害するインターフェロンによって誘発される細胞外溶性タンパク質を特定し,特徴づけること.
この溶解性タンパク質が抗ウイルス活性を発揮するメカニズムを解明する.

主な方法:

28キロダルトンの溶性抗ウイルスタンパク質を同質化まで浄化する.
精製されたタンパク質を識別するためのタンパク質配列.
低密度リポプロテイン受容体 (LDLR) に対するモノクローナル抗体を用いた免疫親和性染色体検査.

主要な成果:

浄化された溶性タンパク質は,ヒトの160キロダルトン低密度リポプロテイン受容体 (LDLR) のリガンド結合ドメインとして特定されました.
VSVに対する溶解性LDLRの抗ウイルス活性が確認されました.
溶解性LDLRは,主にVSVの組み立てや芽生えを妨害することによって,インターフェロンによって誘発された抗ウイルス活動を媒介する.

結論:

低密度リポプロテイン受容体 (LDLR) の溶解形態は,インターフェロンによって誘発された抗ウイルスタンパク質として機能します.
この溶解性LDLRは,VSVに対するインターフェロンの抗ウイルス防御の重要な媒介であり,ウイルスの集合または放出の段階で作用します.

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