ミトのフィードバック制御遺伝子MAD2は,プレニルトランスフェラーゼのアルファサブユニットをコードする
1Department of Biochemistry, University of California, San Francisco 94143-0444.
Nature
|November 4, 1993
まとめ
MAD2遺伝子は,酵母における細胞分裂とタンパク質輸送の両方に不可欠です. 新しい変異体がMAD2を明らかにした.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- MAD2遺伝子は,細胞サイクルフィードバック制御を調節し,酵母細胞が早めにミトーシスから脱出するのを防ぐことが知られている.
- MAD2遺伝子製品は,脂質変異に関与する酵素であるプレニルトランスファーゼと配列の類似性を共有しています.
研究 の 目的:
- タンパク質の密輸におけるMAD2遺伝子産物の機能を調査する.
- MAD2 (mad2-2ts) の新型温度に敏感な変異体の特徴を明らかにする.
主な方法:
- マッド2-2ツ変異体の分離と特徴付け.
- 小型GTP結合タンパク質 (Ypt1pとSec4p) のプレニレーションの測定.
- mad2-2tsとbet2-1変異の間の合成致死性の分析.
主要な成果:
- Mad2pは,プレニル化タンパク質Ypt1pとSec4pの膜結合に不可欠である.
- 変異細胞の抽出物は,基質のゲラニルゲラニル化に欠陥を示しています.
- mad2-2tsとbet2-1の間に観察された合成致死性は,機能的な相互作用を示唆しています.
結論:
- MAD2遺伝子製品は,タンパク質のプレニル化と密輸に不可欠なゲラニルゲラニルトランスファーゼ複合体に関与しています.
- MAD2は,ミトーシス制御とタンパク質輸送経路の両方で明確な役割を果たしています.
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