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関連する概念動画

Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...

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関連する実験動画

Updated: Jun 28, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

スプライス部位の選択に関与するタンパク質と,規制された代替スプライシングの間の特定の相互作用.

J Y Wu1, T Maniatis

  • 1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

Cell
|December 17, 1993
PubMed
まとめ

この研究は,スプライシング因子SC35とSF2/ASFが,U1 snRNPとU2AF35.5と3との相互作用によって,5'および3'スプライシングサイトを橋渡しすることを明らかにしています. これらの相互作用は,プレ-mRNAスプライシングに不可欠であり,ドロソフィラトランスフォーマータンパク質による代替スプライシングを調節する可能性があります.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • mRNA前スプライシングは,5'および3'スプライスサイトの正確な認識とペアリングを必要とします.
  • スプライシングファクターは,この複雑な分子プロセスにおいて重要な役割を果たします.
  • U1小核リボ核タンパク質 (snRNP) は5'スプライス部位に結合し,U2AF35-U2AF65は3'スプライス部位に結合する.

研究 の 目的:

  • 接合因子SC35およびSF2/ASFの接合因子と接合体内の構成要素の相互作用を調査する.
  • スプライスサイト選択と代替スプライス制御におけるこれらの相互作用の役割を明らかにする.

主な方法:

  • 結合パートナーを決定するために,タンパク質-タンパク質相互作用アッセイが使用されました.
  • この研究は,SC35,SF2/ASF,U1 snRNP (U1-70K),U2AF35.5との相互作用に焦点を当てました.

主要な成果:

  • SC35とSF2/ASFはU1-70KとU2AF35.5の両方と特異的に相互作用することが判明しました.
  • これらの相互作用は,5'および3'スプライスサイト間のSRタンパク質のブリッジ機能を示唆しています.
  • また,SC35,SF2/ASF,U2AF35,およびドロソフィラのスプライシングレギュレータであるトランスフォーマー (Tra) とトランスフォーマー-2 (Tra2) の間にも相互作用が観察されました.

さらに関連する動画

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
07:03

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

関連する実験動画

Last Updated: Jun 28, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
07:03

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

結論:

  • SC35とSF2/ASFは, 5'と3'のスプレイスサイト認識装置を接続するブリッジとして機能します.
  • SRタンパク質によって媒介されるタンパク質とタンパク質の相互作用は,スプライス部位の選択に重要である.
  • これらの相互作用は,TraとTra2との相互作用が示すように,代替スプライシングの調節において重要な役割を果たしている可能性が高い.