DUN1は,酵母におけるDNA損傷反応を制御するタンパク質キナーゼをコードする

Z Zhou¹, S J Elledge

¹Howard Hughes Medical Institute, Houston, Texas.

Cell

|December 17, 1993

まとめ

DNA損傷は修復のための遺伝子発現を誘発する. タンパク質キナーゼであるDun1は,このプロセスをリン酸化によって調節し,真核生物のSOS応答を明らかにする.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
遺伝学遺伝学とは

背景:

DNAの損傷は,修復メカニズムを開始するために細胞の反応を活性化します.
遺伝子の発現は,修復能力を強化するために,DNAの損傷後にアップレギュレーションされます.

研究の目的:

DNA損傷によるRNR3遺伝子転写誘導の規制メカニズムを調査する.
DNAダメージ誘発性遺伝子発現に関与する重要な要因と経路を特定する.

主な方法:

DNAダメージを誘導できない (dun) 変異体の分離と特徴付け.
DNA損傷に対する反応として遺伝子誘導パターンの分析.
DUN1遺伝子産物の生化学的特徴.

主要な成果:

DNAダメージを誘導できない変異体 (dun) の5つの補完群が特定され,すべてDNAダメージに敏感である.
dun1変異体はRNR1およびRNR2誘導の欠陥を示したが,他の遺伝子は示さなかったため,異なるDNA損傷誘導経路を示している.
DUN1は核タンパク質キナーゼをコードし,DNA損傷時にリン酸化が増加し,自己リン酸化活性を示唆する.

結論:

この研究では,DUN1がDNA損傷によって引き起こされる遺伝子発現の重要なレギュレータであることを明らかにした.
証拠は,DNA損傷誘導転写のための少なくとも2つの異なる経路の存在を示唆しています.
この発見は,タンパク質のリン酸化によって制御されるエウカリオットSOS応答機構を確立している.

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