ADP-リボシライゼーションファクタは,GTPに依存する小さな調節タンパク質で,フォスフォリファーズDの活性を刺激する
H A Brown1, S Gutowski, C R Moomaw
1Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235-9041.
Cell
|December 17, 1993
まとめ
フォスフォリファーゼD (PLD) の活動は,ADP-リボシライゼーション因子 (ARF) と呼ばれる細胞系因子によって強化されます. このARFタンパク質は,PLDに不可欠です.
科学分野:
- バイオケミストリー バイオケミストリー
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- フォスフォリファーズD (PLD) は,フォスファディチルコリンを水分解して,フォスファディチ酸とコリンを生成します.
- PLDの活動は,サイトゾール成分を含む様々な細胞因子によって調節されます.
- ADP-リボシライゼーション因子 (ARF) は,細胞内膀交通のレギュレータとして知られています.
研究 の 目的:
- 細胞製剤におけるPLD活性を測定するための測定法を開発する.
- PLDの活性を増強する細胞分裂因子を特定し,特徴づけること.
- PLDの調節におけるARFタンパク質の役割を調査する.
主な方法:
- PLD活動のための外部基板ベースの測定法の開発.
- 牛の脳シトゾールからサイトゾール因子を浄化する.
- 生物化学的および分子的技術を用いた精製された因子の特徴化.
- 再結合ミリストイラートARF1.1の発現と検査
主要な成果:
- 膜内のPLD活性を著しく強化する細胞分裂因子が特定されました.
- この因子は精製され,ADP-リボシライゼーション因子 (ARF) のサブファミリーの一員として識別されました.
- 再結合されたミリスチル化ARF1は,非ミリスチル化形態と比較して,PLDの強化活性化を示した.
- ARFタンパク質は,PLDのGTPγSに依存した刺激に不可欠です.
結論:
- ARFタンパク質は,フォスフォリファーズDの活性を調節する上で重要な役割を果たします.
- ARFは,PLDを通じてセカンドメッセンジャーの生成に重要な要素として機能する可能性があります.
- この発見は,ARF媒介の膀の密輸を,PLDを含む信号伝達経路と関連づけています.
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