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Updated: Jul 28, 2026

Comparing the Affinity of GTPase-binding Proteins using Competition Assays
10:37

Comparing the Affinity of GTPase-binding Proteins using Competition Assays

Published on: October 8, 2015

GααサブユニットのGTP結合ドメインとGTPアゼ活性化ドメインを分離する

D W Markby1, R Onrust, H R Bourne

  • 1Department of Pharmcology, University of California, San Francisco 94143.

Science (New York, N.Y.)
|December 17, 1993
PubMed
まとめ

グアノシントリフォスファテーズ (GTPase) スーパーファミリーのほとんどのメンバーは,グアノシントリフォスファート (GTP) を水分解するためにGTPase活性化タンパク質 (GAP) を必要とします. この研究では,Gタンパク質αサブユニットの挿入がGAPとして作用し,GTPase調節のための一般的なメカニズムを示唆していることが示されています.

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Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay13:51

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Published on: November 11, 2018

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G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
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