MyoDまたはMyf-5は,骨格筋の形成のために必要です
M A Rudnicki1, P N Schnegelsberg, R H Stead
1Institute for Molecular Biology and Biotechnology, McMaster University, Hamilton, Ontario, Canada.
Cell
|December 31, 1993
まとめ
MyoDとMyf-5の両方の調節遺伝子を欠いたマウスは,不動で生まれ,出生直後に死亡しており,これらの要因が骨格筋の発達に不可欠であることを示しています. これらの遺伝子は,骨髄形成において余剰的な役割を果たします.
科学分野:
- 発達生物学 発達生物学とは
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- MyoDとMyf-5のようなミオジェニック調節因子 (MRF) は,骨格筋の発達に不可欠です.
- Myf-5またはMyoDのゼロ変異を持つマウスは,正常な骨格筋を示し,機能的な冗長性を示唆しています.
研究 の 目的:
- 骨格筋形成におけるMyoDとMyf-5の機能的な冗長性を調査する.
- 胚の骨格筋形成のためのMyoDとMyf-5の必要性を決定する.
主な方法:
- Myf-5とMyoDの両方の遺伝子が欠けているダブルミュータントマウスの世代.
- ノーザンブロットとS1核酵素アッセイを用いた遺伝子発現の分析.
- 骨格筋の発達に関するヒストロジカルおよび免疫ヒスト化学的検査.
主要な成果:
- Myf-5とMyoDの両方が欠けていたマウスは,生きながらも生まれましたが,不動性と胎内死亡を示しました.
- 二重変異マウスの骨格筋特異的mRNAが検出できない.
- 骨格筋とデスミン発現するミオブラストのような細胞の完全な欠如が観察されました.
結論:
- Myf-5とMyoDは,胚の発達中の骨格のミオブラストの決定および/または増殖に両方必要である.
- これらの要因は,骨格筋の形成に不可欠な,少なくとも部分的に冗長な役割を果たします.
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