関連する実験動画
Updated: May 2, 2026
06:37
Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
Published on: February 7, 2013
16.1K
S. cerevisiaeのFET3遺伝子は,鉄鉄の吸収に必要な多銅酸化酵素をコードする
1Department of Pathology, University of Utah College of Medicine, Salt Lake City 84132.
Cell
|January 28, 1994
まとめ
Saccharomyces cerevisiaeは,鉄の蓄積のためにフェリリデクタゼと鉄素トランスポーターを必要とします. 多銅酸化還元酵素をコードするFET3遺伝子は,酵母における高親和性の鉄吸収に不可欠です.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- 鉄は細胞のプロセスに不可欠であり,鉄の吸収は厳しく規制されています.
- Saccharomyces cerevisiaeは,鉄の蓄積のためにフェリリデクタゼと鉄質トランスポーターを使用します.
- 高親和性のFe (II) 吸収に欠陥のある突然変異体が特定されました.
研究 の 目的:
- S. cerevisiaeにおける高親和性鉄の吸収を司る遺伝子を分離し,特徴づけること.
- 細胞の鉄蓄積におけるFET3遺伝子産物の役割を明らかにする.
主な方法:
- 変異酵母菌株 (fet3) と野生型のFET3遺伝子との補完.
- FET3遺伝子のDNAシーケンシングと分析.
- 異なる銅濃度下での鉄鉄輸送活動の評価.
主要な成果:
- 野生型のFET3遺伝子は,補足によって分離されました.
- 配列分析により,FET3は青色多銅酸化還元酵素に似たタンパク質をコードしていることが明らかになった.
- 銅の欠乏が鉄の輸送を減らし,他の金属ではなく,銅を加えることで復元された.
結論:
- Fet3タンパク質は,銅を含むオキシドリデクタゼであると考えられ,S. cerevisiaeにおける高親和性鉄鉄輸送に不可欠である.
- Fet3の触媒的活動は,効率的な細胞鉄の蓄積に必要とされる可能性が高い.
さらに関連する動画
10:34Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
Published on: February 1, 2018
10.3K
08:36Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth
Published on: May 31, 2024
1.1K
関連する概念動画
01:28The Early Endosome: Endocytosis of Transferrin
4.1K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
4.1K
01:28Microbial Nutrition
2.0K
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
2.0K
01:20Sulfur Assimilation
554
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
554
01:24Microbes and Other Elemental Cycles
89
Microbial activity plays a pivotal role in the biogeochemical cycling of iron and manganese, especially at the redox gradients characteristic of stratified aquatic environments. These cycles are driven by microbial transformations between oxidized and reduced forms of the metals, allowing organisms to exploit them for metabolic energy and structural purposes.Iron Cycling Across Redox GradientsIn neutral, oxygen-rich surface waters, iron is predominantly found in its oxidized, insoluble ferric...
89
01:19Acid Mine Drainage
112
Mining activities that disturb sulfide-rich rocks, particularly those containing pyrite (FeS₂), initiate a cascade of geochemical and microbiological processes with serious environmental implications. When exposed to air and water, pyrite undergoes oxidation, releasing sulfate, ultimately forming sulfuric acid and mobilizing heavy metals into surrounding water systems. This phenomenon, known as acid mine drainage (AMD), results in low pH waters laden with toxic elements that threaten...
112
01:22Bioreactor Controls-III
67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67