酵母菌におけるRNAポリメラーゼII CTDとTATA結合タンパク質に関連した多サブユニット複合体
C M Thompson1, A J Koleske, D M Chao
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142.
Cell
|July 2, 1993
まとめ
研究者らは,RNAポリメラーゼIIカルボキシ末端領域 (CTD) と相互作用する新しいSRBタンパク質 (SRB2,SRB4,SRB5,SRB6) を特定した. これらのSRBタンパク質は,TATA結合タンパク質 (TBP) とともに,効率的な遺伝子転写開始に不可欠な複合体を形成します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- RNAポリメラーゼIIのカルボキシ末端領域 (CTD) は,転写において重要な役割を果たします.
- CTDを含むタンパク質の相互作用を理解することは,転写調節を解読する鍵です.
研究 の 目的:
- RNAポリメラーゼIICTDと相互作用する因子を特定し,特徴づけること.
- 転写開始複合体の構成と機能を明らかにする.
主な方法:
- S. cerevisiaeにおけるCTD断絶変異のエキストラジェニックサプレッサーの遺伝子スクリーニング.
- タンパク質複合体の生化学的分離と特徴付け.
- インビトロ転写とテンプレートコミットメントアッセイ.
主要な成果:
- CTD機能に関与する4つのSRB遺伝子 (SRB2,SRB4,SRB5,SRB6) の特定.
- SRBタンパク質とTATA結合タンパク質 (TBP) を含む大型マルチサブユニット複合体の発見.
- このSRB-TBP複合体がRNAポリメラーゼIICTDと結合し,効率的な転写開始に必要であることを実証する.
結論:
- SRB-TBP複合体は,転写開始機構の不可欠な構成要素である.
- SRB2とSRB5は,機能的なプリイニシテーション複合体の形成に不可欠です.
- これらの発見は,RNAポリメラーゼIIによる遺伝子転写の調節に関する重要な洞察を提供します.
関連する概念動画
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Many viruses self-assemble into a fully functional unit using the infected host cell to...
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The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...


