ドロソフィラモルフォゲンビコイドのダウンレギュレーションは,体受容体媒介の信号伝導カスケードによって行われます
E Ronchi1, J Treisman, N Dostatni
1Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.
Cell
|July 30, 1993
まとめ
ドロソフィラのバイコイド (bcd) モルフォゲンパターンは,体 (torso) 信号によって調節されます. トルソシグナル発信はビコイドをリン酸化し,その転写活性化を低下調節し,前部体のパターン形成を精製する.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- ドロソフィラの前部体のパターンは,ビコイド (bcd) タンパク質の梯度によって確立されています.
- 受容体チロシンキナーゼの体 (tor) 信号は,前極の遺伝子転写を抑制する.
研究 の 目的:
- ビコイド (bcd) アクティベーションと体 (tor) 媒介抑制の基礎となる分子メカニズムを調査する.
- トール信号伝達経路におけるD-rafキナーゼとタンパク質リン酸化の役割を解明する.
主な方法:
- 遺伝子の活性化と抑制を研究するために,BCD結合部位を持つ最小のプロモーターを使用しました.
- D-raf キナーゼ,テールレス,フックベインの必要性を調査した.
- bcdタンパク質機能に対する異質の転写活性化ドメインの影響を評価した.
主要な成果:
- bcdの活性化とtorの抑制の両方が,最小のプロモーターシステムを使用して複製されました.
- トルソ媒介抑制はD-rafキナーゼを必要とし,BCDタンパク質のリン酸化を伴う.
- トールによる抑圧は,以前の仮定に反して,テールレスやフックベインに依存しません.
- bcdに異質なアクティベーションドメインを付加することで,tor-mediated repressionに対する無感性を授与した.
結論:
- トール信号伝導カスケードによるバイコイド形態素のリン酸化は,その転写活性化を下調調節する.
- このメカニズムは,ドロソフィラの発達における前部パターンの精巧な制御を提供します.
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