転写因子E2F1の発現により,静止細胞がS相に突入する

D G Johnson¹, J K Schwarz, W D Cress

¹Section of Genetics, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.

Nature

|September 23, 1993

まとめ

E2F転写因子は細胞増殖に極めて重要です. 過剰発現するE2F1は,成長停止細胞におけるDNA合成を活性化させ,細胞サイクル進行におけるその役割を証明する.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
遺伝学遺伝学とは

背景:

E2F転写因子は細胞増殖制御に関与しています.
E2F結合部位は,増殖反応性遺伝子のプロモーターに存在しています.
E2FとRb複合体のような腫瘍抑制タンパク質は,その活動を抑制します.

研究の目的:

細胞増殖制御におけるE2Fの役割の直接的な証拠を提供するために.
DNA合成と細胞サイクル進行に対するE2F1過剰発現の影響を調査する.

主な方法:

E2F1補完性DNA (cDNA) の過剰発現である.
静止細胞にE2F1cDNAをマイクロインジェクションする.
DNA合成とS相エントリの評価.
トランザクティベーション能力が低下したE2F1ミュータントを利用する.

主要な成果:

E2F1の過剰発現は,成長停止細胞におけるDNA合成を活性化させた.
E2F1cDNAのマイクロインジェクションにより,静止細胞にS相エントリーが誘発される.
DHFRとTKプロモーターをトランザクティブにできないE2F1変異体はS相を誘発できませんでした.

結論:

E2F転写因子は,S相への進行において重要な役割を果たします.
S相進行におけるE2Fの機能は,その転写刺激能力と関連している可能性が高い.

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