Muトランスポゼステトラメア内のモノマー間の労働の分割
T A Baker1, M Mizuuchi, H Savilahti
1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health, Bethesda, Maryland 20892.
Cell
|August 27, 1993
まとめ
Muトランスポザーゼ (MuA) のC端は,DNA再結合に不可欠である. 特定の30アミノ酸の領域は,DNA分裂と鎖移転に不可欠であり,鎖移転には4つのモノマーがすべて必要である.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- Muトランスポゼ (MuA) は,DNA分裂と鎖移転を含む一連のステップを通じてDNA転移を媒介する.
- MuAの機能領域を理解することは,転置のメカニズムの解明に不可欠です.
研究 の 目的:
- Muトランスポーゼ (MuA) の重要な領域を特定し,DNAの分裂と鎖移転を担当します.
- MuAの触媒活性におけるC端の役割を調査する.
主な方法:
- MuAのC端末消去誘導体の分析.
- 野生型および突然変異のMuAタンパク質の活性を in vitroで評価する.
- ワイルドタイプとデレーション変異型MuAで構成された混合テトラメアの形成と分析.
主要な成果:
- MuA C末端にあるA 30アミノ酸領域 (残基575-605) は,DNA分裂と鎖移転に不可欠である.
- この領域が欠けている削除変異体は,独立して無活性であるが,野生型のMuA.と混合テトラマーに組み立てることができる.
- 混合テトラメアはドナーDNAの分裂を起こすが,鎖の移転を促すことはできない.
結論:
- Muトランスポゼーゼの活性の中心は,4つのMuAモノメアのC端から構成されています.
- ドナーDNAの分裂には2つのモノマーが十分であり,鎖の移転には4つのモノマーがすべて必要である.
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