バクテリオファージT4 Alcタンパク質:DNAの局所的な改変を感知する転写終止因子
M Kashlev1, E Nudler, A Goldfarb
1Public Health Research Institute, New York, New York 10016.
Cell
|October 8, 1993
まとめ
バクテリオファージT4 Alcタンパク質は,特定のDNA部位でE. coliの転写を終了させます. 終結はRNAポリメラーゼの接近とE. coliのDNA改変に依存し,T4のDNAではない.
科学分野:
- 微生物学 微生物学とは
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- E. coliのバクテリオファージT4感染は,宿主転写の停止を伴う.
- バクテリオファージT4のAlcタンパク質は,このプロセスに関与しています.
研究 の 目的:
- バクテリオファージT4 Alcタンパク質がE. coliの転写を終了させるメカニズムを解明する.
- アルコール依存性終結とT4の抵抗に影響を与える要因を調査する.
主な方法:
- サイト・スペシフィック・ターミネーションアッセイ in vitro.
- RNAポリメラーゼの停止とE. coliのDNA改変の分析.
- T4DNAにおけるサイトシン改変の役割を調査する.
主要な成果:
- Alcは,E. coliのサイト固有の転写終結因子として機能する.
- 終結は,アルク部位近くのRNAポリメラーゼの一時的な停止によって防止されます.
- バクテリオファージT4DNAは,サイトシン改変 (5-ヒドロキシメチルサイトシン) によってアルクから保護されています.
- Alcの近くのCpG部位のインビトロメチル化により,その終止効果は廃止されました.
結論:
- ALC依存終結は,DNAのサイトシン改変状態を感知することを含む.
- このプロセスは,最近のRNAポリメラーゼの一時停止の短期記憶を示します.
- バクテリオファージT4は,Alc.によってホストの転写停止を回避するためにDNAの改変を採用します.
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